Fig. 8: Verification of Beclin-1 S-nitrosation function and targeting GSNOR in iWAT to combat middle-aged obesity. | Nature Communications

Fig. 8: Verification of Beclin-1 S-nitrosation function and targeting GSNOR in iWAT to combat middle-aged obesity.

From: S-nitrosoglutathione reductase GSNOR drives age-related obesity by promoting adipose tissue whitening through de-nitrosation of Beclin-1

Fig. 8: Verification of Beclin-1 S-nitrosation function and targeting GSNOR in iWAT to combat middle-aged obesity.The alternative text for this image may have been generated using AI.

a Oil Red O staining of primary adipocytes from WT and Beclin-1C351A mice treated with sh-ctrl or shGSNOR adeno-associated virus (AAV) at 4 days of rosiglitazone withdrawal. Scale bar, 50 μm. (n represented the number of lipid droplets. WT untreated (n = 15), WT treated (n = 16), Beclin-1 C351A untreated (n = 17), Beclin-1 C351A treated (n = 14). Representative data from three independent replicates experiments). b Immunoblotting for UCP1 protein levels in primary adipocytes from WT and Beclin-1C351A mice infected with sh-ctrl or shGSNOR AAV. (Representative data from three independent replicates experiments). c Schematic illustration of the site-directed AAV injections used to knockdown GSNOR expression in the iWAT of 14-month-old mice. d Immunoblotting for GSNOR and UCP1 protein levels and densitometric quantification of the bands (n = 7). e Gross view and weight of iWAT injected with control (AAV-sh-ctrl) or AAV-shGSNOR (n = 11). f H&E staining of iWAT from mice injected with control (AAV-sh-ctrl) or AAV-shGSNOR (n = 6). Scale bar, 200 μm. g A graph model depicting the role of GSNOR in age-related obesity. Data are represented as mean ± SD; Significance was assessed by Unpaired two-tailed Student’s t test (a, b), Paired two-tailed Student’s t test (d–f) (*p < 0.05, ***p < 0.001, ns for not significant). AAV adeno-associated virus, H&E iWAT, H&E hematoxylin and eosin, iWAT inguinal white adipose tissue.

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