Table 1 List of CCDC6-RET phosphopeptides (phospho-sites in bold) identified in at least two out of four independent autophosphorylation experiments, see also Supplementary Tables 1–4, (*) autophosphorylation sites. Phospho-peptides identified with miscleavages and different charge states are shown
From: The oncogenic CCDC6-RET fusion protein is a dual ATP- and ADP-dependent kinase
Peptide sequence | Protein name | Phosphosite (autoP*) |
|---|---|---|
SGGIVISPFR (+2) SGGIVISPFRLEELTNR (+2) (+3) | CCDC6 | S46* |
SGGIVISPFR (+2) SGGIVISPFRLEELTNR (+2) (+3) SGGIVISPFRLEELTNRLASLQQENK (+3) (+4) | CCDC6 | S52* |
LASLQQENK (+2) LASLQQENKVLK (+2) | CCDC6 | S65 |
LASLQQENKVLKIELETYK (+3) (+4) VLKIELETYK (+2) (+3) VLKIELETYKLK (+2) (+3) (+4) IELETYK (+2) IELETYKLK (+2) (+3) IELETYKLKCK (+2) (+3) (+4) | CCDC6 | Y80* |
KASVTIEDPK (+2) KASVTIEDPKWEFPR (+2) (+3) (+4) ASVTIEDPKWEFPRK (+3) ASVTIEDPK (+2) | CCDC6 | S98* |
QVNHPHVIKLYGACSQDGPLLLIVEYAK (+4) LYGACSQDGPLLLIVEYAK (+2) (+3) | RET | Y791* |
QVNHPHVIKLYGACSQDGPLLLIVEYAK (+4) LYGACSQDGPLLLIVEYAK (+2) (+3) | RET | Y806* |
YGSLRGFLR (+2) (+3) YGSLRGFLRESR (+3) | RET | Y809* |
ESRKVGPGYPDER (+2) (+3) KVGPGYPDER (+2) (+3) VGPGYPDER (+2) KVGPGYPDERALTMGDLISFAWQISQGMQYLAEMK (+4) | RET | Y826* |
ISDFGLSRDVYEEDSYVK (+2) (+3) ISDFGLSRDVYEEDSYVKR (+2) (+3) (+4) DVYEEDSYVK (+2) DVYEEDSYVKR (+2) (+3) MKISDFGLSRDVYEEDSYVK (+3) | RET | Y900 |
MKISDFGLSRDVYEEDSYVK (+3) ISDFGLSRDVYEEDSYVK (+2) (+3) ISDFGLSRDVYEEDSYVKR (+4) DVYEEDSYVK (+2) DVYEEDSYVKR (+2) (+3) DVYEEDSYVKRSQGR (+3) | RET | Y900/Y905 * |
DVYEEDSYVKRSQGR (+3) DVYEEDSYVKR (+2) (+3) | RET | S904* |
ISDFGLSRDVYEEDSYVK (+2) (+3) ISDFGLSRDVYEEDSYVKR (+2) (+3) (+4) DVYEEDSYVK (+2) DVYEEDSYVKR (+2) (+3) | RET | Y905* |
SQGRIPVK (+2) RSQGRIPVK (+2) (+3) | RET | S909* |
RRDYLDLAASTPSDSLIYDDGLSEEETPLVDCNNAPLPR (+3) (+4) RDYLDLAASTPSDSLIYDDGLSEEETPLVDCNNAPLPR (+3) (+4) DYLDLAASTPSDSLIYDDGLSEEETPLVDCNNAPLPR (+3) (+4) | RET | Y1015* |
RRDYLDLAASTPSDSLIYDDGLSEEETPLVDCNNAPLPR (+4) RDYLDLAASTPSDSLIYDDGLSEEETPLVDCNNAPLPR (+3) (+4) | RET | Y1015 + Y1029* |
RRDYLDLAASTPSDSLIYDDGLSEEETPLVDCNNAPLPR (+4) RDYLDLAASTPSDSLIYDDGLSEEETPLVDCNNAPLPR (+3) (+4) | RET | Y1015* + S1034 |
RRDYLDLAASTPSDSLIYDDGLSEEETPLVDCNNAPLPR (+4) RDYLDLAASTPSDSLIYDDGLSEEETPLVDCNNAPLPR (+3) (+4) | RET | Y1029* + S1034 |
RRDYLDLAASTPSDSLIYDDGLSEEETPLVDCNNAPLPR (+3) (+4) RDYLDLAASTPSDSLIYDDGLSEEETPLVDCNNAPLPR (+3) (+4) DYLDLAASTPSDSLIYDDGLSEEETPLVDCNNAPLPR (+3) (+4) | RET | S1034 |
ALPSTWIENKLYGR (+2) (+3) LYGRISHAFTRF (+2) (+3) (+4) | RET | Y1062* |
