Fig. 4: STOP1 functions genetically downstream of BRM in response to low pH.
a, b Root growth of Col-0, stop1, brm, and stop1 brm double mutants. Three-day-old seedlings were transferred to either neutral or low-pH medium and grown for an additional 3 days (n = 20 seedlings). Scale bar: 1 cm. c, d Nitrate uptake rates and relative expression of NRT1.1 in Col-0, stop1, brm, and stop1 brm mutants under neutral and low-pH conditions. Data are shown as mean ± SD (n = 4 biological replicates per genotype and condition). In (b, d), centerlines in the boxplots show the medians, box limits indicate the 25th and 75th percentile, and whiskers indicate the minimum and maximum values. In (c), data represent mean ± SD. Different letters indicate statistically significant differences between means (p < 0.05), determined by Tukey’s multiple comparisons test (one-way ANOVA in (b, d); two-way ANOVA in (c)). Exact p values are provided in the Source Data file. All experiments were repeated at least three times independently, with similar results.
