Fig. 6: Donor MHCII-mediated indirect antigen-presentation was required to maintain the pools of stem-like Tm and their progeny in GVHD target tissues.

a Donor derived CD11b⁺ CD11c⁺ APCs gated from H2Kb⁺TCRβ⁻ cells from the lung of No GVHD and cGVHD mice were examined at day 60 after HCT, expression of MHCII from APCs was also measured, N = 4. b,c cGVHD mice were established with CD45.2⁺ WT or MHCII^−/− CD45.2⁺ BALB/c recipients given C57BL/6 CD45.2⁺ WT-BM or MHCII^−/−- BM plus CD45.1⁺ WT-T cells. Four Tm subsets among injected donor-type (CD45.1⁺) CD4⁺ T cells from the lung (b) and liver (c) were examined on days 7 (N = 3 or 4 per group) and 60 after HCT (N = 6, 4 or 6 per group). d Proinflammatory IFN-γ and GM-CSF production among Trm cells was measured on day 60 after HCT (N = 6 per group). e BALB/c recipients were established with WT-T plus WT or IFNγR^−/− TCD-BM. %survival and %baseline bodyweight. N = 10–15. f Representative Masion’ trichrome staining panel and pathology scores are shown (magnification, x200, N = 4). g Representative contour plots, percentages and yields of the four Tm subsets were analyzed on day 60 after HCT, N = 4 ~ 6. h Proinflammatory IFN-γ and GM-CSF production among CD4⁺ Tm cells, N = 4. i Percentages of CD11b⁺ CD11c⁺ APCs were also compared, and MHCII expression was analyzed, N = 4 ~ 6. Data presented as mean ± SEM from at least 2 replicates. *P < 0.05, **P < 0.01, ***P < 0.001, ****P < 0.0001, as determined by two-way ANOVA (a,b,f,g), one-way ANOVA (d), log-rank test comparison of survival (e) and unpaired two-tailed t-test (e,h,i).