Fig. 5: PB@PM improves cardiac function and attenuates post-infarct remodeling. | Nature Communications

Fig. 5: PB@PM improves cardiac function and attenuates post-infarct remodeling.

From: Prussian blue nanoparticles targeting multiple PANoptosome-mediated PANoptosis for myocardial ischemia-reperfusion injury therapy

Fig. 5: PB@PM improves cardiac function and attenuates post-infarct remodeling.

A–E Echocardiographic analysis of left ventricular ejection fraction (LVEF), LV fraction shortening (LVFS), LV end-systolic volume (LVESV), and LV end-diastolic volume (LVEDV) on preoperative day 1 and postoperative days 1, 14, and 28. Scale bar: 100 ms. Exact P-values from upper to lower: (B) day 14 (0.0012), day 28 (0.00003, 0.0028); (C) day 14 (0.0056), day 28 (0.0002, 0.039); (D) day 14 (0.0011, 0.0201), day 28 (0.00001, 0.0004); (E) day 14 (0.015, 0.0294), day 28 (0.0101). F Representative Masson and HE staining in hearts on 28 days post-surgery. Scale bar: 1 mm. G Fibrosis quantification in hearts 28 days post-surgery. Exact P-values from upper to lower: 2.28E-08, 3.28E-07, 0.0116. H Evans blue/TTC staining and quantitative analysis showing the cardiac cell survival and blood perfusion in different groups on day 3 post-surgery. AAR: area at risk. IF: infarct zones. Scale bar: 1 mm. Exact P-values from upper to lower: (AAR/LV) 0.4990 for MIRI vs. PB, 0.3119 for MIRI vs. PB@PM, 0.9269 for PB vs. PB@PM; (IF/AAR) 3.48E-08, 0.0001, 0.00002. I, J TUNEL (red) and cTnT (green) co-staining and quantification in peri-infarct zones (day 3). Scale bar: 50 μm. Exact P-values from upper to lower: 0.000005, 0.0006, 0.0154. K, L Representative immunofluorescence images and quantitative analysis of CD86 (red) in peri-infarct regions (day 3). Scale bar: 1 mm or 50 μm. Exact P-values from upper to lower: 2.17E-08, 0.00001, 0.00005. M, N Representative immunofluorescence staining and quantitative analysis of Ly-6G (red) in peri-infarct regions (day 3). Scale bar: 1 mm or 50 μm. Exact P-values from upper to lower: 8.27E-09, 5.89E-08, 9.99E-07. O–R Representative immunofluorescence staining and quantification analysis of α-SMA (red)/cTnT (green) and CD31(red)/cTnT (green) in peri-infarct region (day 28). Scale bar: 1 mm or 50 μm. Exact P-values from upper to lower: (P) 0.000002, 0.0005, 0.0026; (R) 0.000004, 0.0003, 0.016. Data: mean ± SD of 5 biologically independent replicates. Significance: one-way ANOVA with Tukey’s post-hoc test (*P < 0.05, **P < 0.01, ***P < 0.001; ns, P > 0.05). Source data are provided as a Source Data file.

Back to article page