Fig. 2: Transcription orientation affects orientation-specific CSR.
From: Chromatin-intrinsic mechanisms determine orientation-specific class switch recombination
a Schematic of the Igh locus from V(D)J exon to 3′CBEs in CH12F3PO cells and an illustration of the generation of αInv cells. Joining features of Sμ–Sα junctions in CH12F3PO (b) and αInv (c) cells. Data are presented as mean ± s.e.m. from three independent experiments. d Bar graph shows the inversional joining of Sμ–Sα junctions in CH12F3PO and αInv cells. Data are presented as mean ± s.e.m. from five independent experiments. e Schematic of the Igh locus from the V(D)J exon to 3′CBEs in IαΔ cells and an illustration of the generation of γ2aInv cells. Joining features of Sμ−Sγ2a junctions in IαΔ (f) and γ2aInv (g) cells. h Bar graph shows the inversional joining of Sμ−Sγ2a junctions in IαΔ and γ2aInv cells. i Schematic of the Igh locus from V(D)J exon to 3′CBEs in IαΔ cells and illustration of the generation of γ1Inv cells. Joining features of Sμ−Sγ1 junctions in IαΔ (j) and γ1Inv (k) cells. l Bar graph shows the inversional joining of Sμ−Sγ1 junctions in IαΔ and γ1Inv cells. m Schematic of Igh locus from V(D)J exon to 3′CBEs in γ3-αInv cells and illustration of the generation of γ3-αInv-αInv cells. Joining features of Sμ–Sα junctions in γ3-αInv (n) and γ3-αInv-αInv (o) cells. p Bar graph shows the inversional joining of Sμ–Sα junctions in γ3-αInv and γ3-αInv-αInv cells. For (f–h), (j–l), and (n–p), data are presented as mean ± s.e.m. from three independent experiments. P-values were calculated via an unpaired two-tailed Student’s t-test. Source data are provided as a Source Data file.
