Fig. 4: Whole-lung mRNA expression demonstrates that endothelial CNP/GC-B signaling regulates vasoactive and inflammatory genes in mouse models of pulmonary hypertension. | Nature Communications

Fig. 4: Whole-lung mRNA expression demonstrates that endothelial CNP/GC-B signaling regulates vasoactive and inflammatory genes in mouse models of pulmonary hypertension.

From: Endothelial C-type natriuretic peptide/guanylyl cyclase-B signaling prevents pulmonary arterial hypertension

Fig. 4: Whole-lung mRNA expression demonstrates that endothelial CNP/GC-B signaling regulates vasoactive and inflammatory genes in mouse models of pulmonary hypertension.

RT-qPCR analyses of the whole lung expression of Nppc (a, b), Npr2 (c, d), Npr1 (e, f), Edn1 (g, h), Il6 (i, j), Ccl2 (k, l), and Tgfb1 (m, n) in Tie2-Cre, SM22α-Cre, CNP ecKO, GC-B ecKO and GC-B smcKO mice in the MCTp-induced model (10-week-old, a, c, e, g, i, k, m) and Hx-induced model (12-week-old, b, d, f, h, j, l, n) compared with untreated controls (10-week-old of MCTp (–) and 12-week-old normoxia; n = 8 per group). GAPDH was used as the internal control. Data are presented as the mean ± SEM. P values were calculated using one-way ANOVA with Tukey’s multiple comparisons test.

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