Fig. 2: Single-Cell Multi-Omics Analysis Reveals Elevated Mechanical Stress in ECs of the SiO2 - Induced Mouse PF Model. | Nature Communications

Fig. 2: Single-Cell Multi-Omics Analysis Reveals Elevated Mechanical Stress in ECs of the SiO2 - Induced Mouse PF Model.

From: Single-cell multiomics uncovers an endothelial mechanosensitive PIEZO1-IL-33 axis driving pulmonary fibrosis

Fig. 2: Single-Cell Multi-Omics Analysis Reveals Elevated Mechanical Stress in ECs of the SiO2 - Induced Mouse PF Model.

A Schematic diagram of the SiO2 mouse model. Mice were divided into control (saline, n = 3) and experimental (SiO2, n = 3) groups by tracheal injection. After 20 weeks, mice were euthanized, and lung single-cell suspensions were prepared for sequencing. B Validation of the SiO2 mouse model. H&E and Masson staining showed increased inflammation and fibrosis in the lungs of SiO2 treated mice (n = 3 mice per group). C UMAP plots of lung tissue scRNA-seq data from SiO2-induced and control mice. D UMAP plots of lung tissue scATAC-seq data from SiO2-induced and control mice. E Violin plots showed expression of marker genes of scRNA-seq data from SiO2-induced and control mice. F In scATAC-seq data from SiO2-induced and control mice, peaks for marker genes were identified for each subcelltype. G The UMAP plot showed endothelial subpopulations in scRNA-seq data from SiO2-induced and control mice. UMAP plot (H) and violin plot (I) showed the MS scores of endothelial cells in scRNA-seq data of Silica-induced (n = 784 cells) and control (n = 816 cells) mice. J The UMAP plot showed the endothelial subpopulations of scATAC-seq data. UMAP plot (K) and violin plot (L) showed the MS scores of endothelial cells in scRNA-seq data of Silica-induced (n = 387 cells) and control (n = 689 cells) mice. M GO enrichment analysis of motifs showed that signaling pathways associated with mechanical stress were enriched in ECs. Statistical significance of GO terms was calculated using the hypergeometric test. N Table showing TF motifs of endothelial cells enriched in mechanical stress-related pathways by scATAC-seq data. P values were calculated using the hypergeometric test. I, L: Violin plot displayed the mean (yellow diamond) the median (center line), the interquartile range, and the whiskers (1.5 × interquartile range). P value was calculated using the two-tailed unpaired t-test. Source data are provided as a Source Data file.

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