Fig. 3: Target validation of cis-compounds 20 and 21 in C. albicans cells.
From: Inhibiting Mrt4-rRNA interaction with fumaramidmycin-based derivatives as an antifungal strategy
a Sensitivity correlation of C. albicans strains to 20 and 21 based on the MRT4 gene dosage and mutation status. The growth of wild-type and mutant strains was monitored by measuring the optical density (OD600) over time in liquid cultures. The data represent the mean of three independent experiments. b Schematic representation of the Mrt4-associated pathway involved in the assembly of the ribosome stalk base in eukaryotes. Created in BioRender. Wang, W. (2026) https://BioRender.com/z9cwodr. c Top pathways affected by 20 treatment in C. albicans SC5314. The scatter plot shows the number of differentially expressed genes (DEGs) as a function of point size, while the color gradient (from blue to yellow) indicates increasing enrichment significance. d Global gene expression changes in C. albicans SC5314 following treatment of 20. Fold changes were determined by comparing normalized transcript counts between 20-treated samples and DMSO-treated controls. A one-sided version of Fisher’s exact test was performed, and the p-value was adjusted using the Benjamini–Hochberg method. e Relative transcriptional changes of MRT4 and related genes (YVH1 and RPL12) between the DMSO-treated group and 20-treated group. f Polysomal profiles of C. albicans SC5314 cells treated with DMSO (control) or 20. The data are representative of three independent experiments. g Quantitative comparison of the peak areas for ribosomal mono-subunits (40S, 60S, and 80S) between the DMSO-treated and 20-treated groups. The data are representative of three biological replicates. Data are presented as the means ± SDs (n = 3 independent experiments). Statistical significance was determined using a two-tailed unpaired Student’s t-test. Source data are provided as a Source data file for (e, f, g).
