Fig. 8: Osteopontin and lipocalin 2 levels represent biomarkers for wound healing after TBI.
From: NF-κB activation in astrocytes impairs wound healing after traumatic brain injury in male mice
a Heat map (log2 normalization) of genes encoding inflammasome components expressed in GoF ACSA-2+ astrocytes at day 3 post-TBI compared to TBI controls. b Immunoblot analysis and corresponding quantification of c ASC, d NLRP3 and e AIM2 inflammasome components. Expression was normalized to eIF2 loading control. f Immunoblot analyses and the corresponding quantification of protein expression of g TGM1, h NRF2, i IBA1, j HO-1, k MAC2, l OPN and m LCN2 in the ipsilateral insult region (ip) and in the contralateral uninjured (co) cortical brain tissue from control (n = 3) and IKK2-CAGFAP (n = 3) animals. Tissue was isolated at day 3 post-TBI. n Ratio of normalized OPN and LCN2. For quantification, protein levels were normalized to β-actin loading control. Bars indicate mean ± SEM. Statistical comparison by ordinary one-way ANOVA with Tukey’s multiple comparisons test. Exact significances indicated in graphs. Protein expression presented in arbitrary units (arb. units). Protein data are presented as biological replicates. Blue: control ipsi, white: control contra, red: IKK2-CAGFAP ipsi, black: IKK2-CAGFAP contra. OPN: Osteopontin (Spp1), HO-1: Heme oxygenase 1 (Hmox1), MAC2: Galectin-3 (Lgals3). Source data are provided as a Source data file.
