Fig. 3: Increased cholinergic-mediated striatal serotonin release in Sapap3 KO mice.
From: Synchronous activation of striatal cholinergic interneurons induces local serotonin release
a Representative line scan traces of GRAB-5HT fluorescence in the dorsal striatum of wild type (WT) (top, black) and knockout (KO) Sapap3 (bottom, blue) mice, before and after bath application of mecamylamine (mec, magenta). b Distribution of peak GRAB-5HT responses before (black – WT, cyan - KO) and after (magenta) application of mecamylamine in WT (left, N = 5 mice, n = 9 slices t16 = –3.5054, P = 0.002, LMEM) and KO (right, N = 6 mice, n = 12 slices t22 = –6.066, P = 4.2∙ 10-6 LMEM). c Quantification of mec-sensitive (left, t19 = 2.89, P = 0.009) and -insensitive (right, t19 = 9.5∙ 10-5 P = 0.999, LMEM) components of GRAB-5HT signal in WT and KO mice. Boxplots indicate: range (whiskers), median (thick bar) and 25 and 75th percentiles (box). * P < 0.05; ** P < 0.01; *** P < 0.001; n.s – not significant. ACSF – artificial cerebrospinal fluid.
