Fig. 6: Anti-CD47 treatment shows decreased cancer cell number, increase in phagocytosis and wobbling macrophages in the OvCAR3 pentaculture. | Nature Communications

Fig. 6: Anti-CD47 treatment shows decreased cancer cell number, increase in phagocytosis and wobbling macrophages in the OvCAR3 pentaculture.

From: 3D pentaculture model unveils malignant cell-driven macrophage polarization in high-grade serous ovarian cancer

Fig. 6: Anti-CD47 treatment shows decreased cancer cell number, increase in phagocytosis and wobbling macrophages in the OvCAR3 pentaculture.The alternative text for this image may have been generated using AI.

A Schematic of the “do-not-eat-me” signal pathways. Created in BioRender. Malacrida, B. (2026) https://BioRender.com/ur79c5g. Percentage of remaining viable cancer cells and of macrophage positive for cancer cells in (B) AOCS1 and (C) G164 pentacultures treated with anti-CD47 mAb. Data presented as mean ± SEM (n = 3 biological replicates using 3 different donors of the primary cells). D IF snapshots from live recordings of the G164 pentaculture. CD45/CD14 in green, FN1 in blue, CD90 in purple, malignant cells in red. Scale bar 50 µm. E Percentage of remaining viable cancer cells and of macrophage positive for cancer cells in OvCAR3 pentaculture. Data presented as mean ± SEM (n = 3 biological replicates using 3 different donors of the primary cells). Paired two-tailed t-test. F IF snapshots from live recordings of the OvCAR3 pentaculture. CD45/CD14 in green, FN1 in blue, CD90 in purple, malignant cells in red. Scale bar 50 µm. G Average percentage of macrophages at a distance less than 20 µm from malignant cells. Data presented as mean ± SEM of 5 frames. Unpaired two-tailed t-test (n = 2 in Supplementary Fig. 6 G, biological replicates using 2 different donors of the primary cells). H Proportions of the different types of behavior (blue, static; beige, wobbling; light grey, migrating; light blue, long migrating), I percentage of the wobbling macrophages, J Trajectory straightness and speed of the wobbling macrophages in the OvCAR3 pentacultures. Data shown as mean ± SEM (n = 2). K Percentage of remaining cancer cells and of macrophage positive for cancer cells in G164 pentacultures control and treated with TGFβRi. Data shown as mean ± SEM (n = 4 biological replicates using 3 different donors of the primary cells). Paired two-tailed t-test. L Percentage of viable cancer cells on G164 tetraculture after 7 days. Data shown as mean ± SEM (n = 3 biological replicates using 3 different donors of the primary cells). M ELISA assay results showing the release of TNFα in the G164 pentacultures control and after TGFβRi treatment. Data shown as mean ± SEM (n = 3 biological replicates using 3 different donors of the primary cells). Paired two-tailed t-test. Source data are provided as a Source Data file.

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