Fig. 2: Highly expressed putative kleptoplast genes and their time-series transcript level changes.

a Thirty-seven R. viridis transcripts encoding well-predicted full-length domains associated with putative chloroplast functions³⁷ (solid bars), along with six mitochondria-targeted transcripts corresponding to components of complex III and the pyruvate dehydrogenase complex (open bars), all exhibited similarly high maximum expression levels. b Normalized transcript counts across four timepoints in a time-series transcriptome dataset. The vertical axis shows normalized counts (mean; n = 3 biological replicates per time point) scaled to the maximum value of each gene; the horizontal axis marks the sampling points corresponding to the early and late kleptoplast transformation phases and two later timepoints in the growth and stationary phases of the phototrophic stage. The genes are grouped according to the timepoint of peak expression. c RNA-seq counts and qPCR measurements for RvRbcS-like (black) and RvRca-like (red). Solid squares with error bars represent normalized RNA-seq counts (mean ± SEM; n = 3 biological replicates per time point; left axis), and the solid circles represent normalized qPCR values (single measurement per time point; n = 1; right axis). qPCR confirmed that RvRbcS-like peaked during the late kleptoplast transformation phase, whereas RvRca-like peaked slightly later, between the late kleptoplast transformation phase and the growth phase. Source data are provided as a Source data file.