Fig. 5: Reporter assays testing the NtLCDs of R. viridis nuclear-encoded proteins as kleptoplast-targeting signals. | Nature Communications

Fig. 5: Reporter assays testing the NtLCDs of R. viridis nuclear-encoded proteins as kleptoplast-targeting signals.

From: Transient molecular chimerism for exploiting xenogeneic organelles

Fig. 5: Reporter assays testing the NtLCDs of R. viridis nuclear-encoded proteins as kleptoplast-targeting signals.

a Gene construct used to induce the expression of codon-optimized luciferase (optNluc) and the G418 resistance marker gene (neor), flanked by the E. gracilis pyruvate: NADP(+)-oxidoreductase gene (EgPNO) promoter and the Arabidopsis thaliana heat shock protein gene (AtHSP) terminator. b Gene construct incorporating an NtLCD sequence from RvRbcS-like (519 bp) upstream of the optNluc-neor cassette. c Bioluminescence microscopy of R. viridis transformant expressing luciferase without an NtLCD sequence, showing cytosolic bioluminescence spatially separated from chlorophyll fluorescence. d Bioluminescence microscopy of the transformant expressing luciferase fused to the NtLCD of RvRbcS-like, showing overlapping bioluminescence and chlorophyll fluorescence, indicating localization to the kleptoplast.

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