Fig. 5: SPF and gnotobiotic mice colonized with Prevotella spp produce SCFA from inulin in vivo.
From: Small intestinal microbial fiber metabolism dysfunction in celiac disease
a Schematic protocol for diet intervention in non-sensitized germ-free (GF, squares) and specific pathogen-free (SPF, circles) NOD/DQ8 mice were fed either GFD with no added fiber (gray) or supplemented with inulin (blue) for 3 weeks (n = 5/group). b Small intestinal SCFA after 3 weeks of no added fiber GFD (white) or inulin diet (blue) in GF (squares) and SPF (circles) mice. Multiple groups were compared using one-way ANOVA followed by Tukey’s test. Individual SI AA GF + NAF vs SPF+Inulin p = 0.00009; GF+Inulin vs SPF+Inulin p = 0.00021). c Schematic protocol for Prevotella colonization of GF NOD/DQ8 mice (n = 4/group). Mice were given inulin diet and colonized by oral gavage with either Prevotella cocktail (triangles) or saline (squares; vehicle control) on days 1 and 3. d Small intestinal SCFA of GF (square) and Prevotella spp. (triangles) colonized mice after three weeks of diet. Data for total SI SCFA was compared using a 2-tailed Students t Test and data for individual SI SCFA were compared using two-way ANOVA followed by Šidák’s multiple comparison test. Individual SI AA GF+Inulin vs P+Inulin p = 0.0002. AA Acetic acid, PA Propionic acid, BA Butyric acid.
