Fig. 7: Host interactome and transcriptome responses to KERP2. | Nature Communications

Fig. 7: Host interactome and transcriptome responses to KERP2.

From: A multifaceted model of Entamoeba histolytica KERP2 regulating gene expression and host cell responses

Fig. 7: Host interactome and transcriptome responses to KERP2.The alternative text for this image may have been generated using AI.

a Anti-HA IP-MS of HA-KERP2-exposed in Caco-2 cells (three biological replicates). Venn shows significant interactors: double hits ( ≥ 2-fold vs HA-mock in ≥2/3 replicates, padj <0.05) and triple-hits (meeting the criterion in all three). b GO Molecular Function enrichment of the combined double-/triple-hit list. Bubble plot: x-axis = GeneRatio; bubble size = gene count; color = FDR. c Heat map of representative interactors (columns) across HA-KERP2, HA-KERP2Δ185–239, and HA-mock IPs (rows). Values are QV intensities. Annotation bars indicate hit type (double/triple) and manual functional grouping (cell-cycle & proliferation; cytoskeletal dynamics; cell–cell/ECM adhesion; intracellular trafficking & vesicle transport; immune/stress; gene regulation & transcription). d Contrast-focused PCA. Genes from the HA-KERP2 vs psAP-KERP2gs contrast (DESeq2, adjusted p < 0.05, |log2FC | ≥ 1) were passed to PCA analysis, showing condition-specific clustering. e Gene-wise comparison of differential expression for HA-KERP2 (x-axis, log2FC) versus psAP-KERP2gs (y-axis, log2FC). Points are colored by significance (padj <0.05): in HA-KERP2, in KERP2gs, or significant in both; exemplar genes are labeled. f, g Preranked GSEA for the HA-KERP2 vs KERP2gs contrast. Genes were ranked by signed –log10(padj) (sign = sign of log2FC); positive NES indicates enrichment in HA-KERP2 and negative NES indicates enrichment in KERP2gs. Only sets with 10–500 genes and FDR q < 0.25 are plotted. f Hallmark collection. g GO Biological Process. Bubble size = gene-set size; color = –log10(FDR).

Back to article page