Fig. 6: Anti-Pfs25 antibodies completely block malaria transmission in the presence of anti-SR-A antibodies.

a Experimental design. After mice were infected with P. berghei-pfs25 transgenic parasites for 3 days, mice were treated with or without CL, and injected with anti-Pfs25 antibodies one hour before fed to mosquitoes. Oocysts number of mosquitoes were counted 9 days post-blood meal. b–e After pretreated with or without CL, the P. berghei-pfs25-infected mice were then inoculated with anti-Pfs25; both the infection intensity (b) and prevalence (c) of mosquitoes, as well as the transmission reduction activity (d) and transmission blocking activity (e), were calculated. f Experimental design. After mice were infected with P. berghei-pfs25 transgenic parasites for 3 days, mice were treated with or without anti-SR-A, and injected with anti-Pfs25 antibodies one hour before fed to mosquitoes. Oocysts number of mosquitoes were counted 9 days post-blood meal. g, h After pretreated with or without anti-SR-A, the P. berghei-pfs25-infected mice were then inoculated with anti-Pfs25, both the infection intensity (g) and prevalence (h) of mosquitoes, as well as the transmission reduction activity (i) and transmission blocking activity (j), were calculated based on infection intensity and prevalence. k–m The relative expression of E. anophelis, or S. sonnei and K. oxytoca in the midgut of mosquitoes was detected by qPCR at 12 h post-feeding on infected mice pre-treated with anti-SR-A, anti-Pfs25 or both. Twenty mosquitoes were pooled as one biological replicate, and three biological replicates were performed. The individual dot in panels of (b, g) represents oocysts number per mosquito, with a line indicating the median, and the pi chart under each scatter chart indicates the sample size and prevalence in each group. Data were presented as mean ± SD (k–m). Three independent experiments were performed for each experiment, and the data were pooled. Source data are provided as a Source Data file. Significance was determined by GLMMs (two-sided) test for (b, g), and Fisher’s exact test (two-sided) was applied for (c, h); an unpaired t-test (two-tailed) was applied for (k–m). Number between each group represent the P-value, P < 0.05 was consider as statistically significant.