Fig. 6: Natural variation in temperature-responsive hypocotyl elongation and ARF protein accumulation.

a Map of locations where accessions originated. b Representative images of 6-day-old seedlings grown at 22 °C or 28 °C. c Immunoblot analysis of seedlings incubated for four hours at 22 °C or 37 °C. Satellite Data accessed from Wolfram Version 14.3 using GeoListPlot. d Mean (± SEM) increase in hypocotyl lengths of 6-day-old seedlings grown at 28 °C versus 22 °C (n = 16 for Wa-1, 19 for Ha-0, 24 for Qui-0, 21 for Noveg-3, 28 for Ca-0, 24 for MNF-Che-47, 18 for Sq-a, 18 for Ler-1, 23 for Map-35, 19 for Sf-2, 27 for Edi-1, 28 for Xan-1, 27 for Hovdala-2, 23 for Sq-1, 24 for Col-0, and 22 for Ciste-2). Color of bar corresponds to fold change measured in immunoblot analysis. e Linear regression comparing ARF7 protein level increase (X-axis) to increase in hypocotyl elongation (Y-axis) at 28 °C relative to 22 °C. Slope determined to be non-zero with a p-value of 0.0025.