Fig. 4: Gemcitabine induces bone marrow myeloid progenitors in a preclinical setting in vivo. | Nature Communications

Fig. 4: Gemcitabine induces bone marrow myeloid progenitors in a preclinical setting in vivo.

From: Chemotherapy-induced activation of caspase-1 and IL-1α release by cancer cells remotely skews myelopoiesis to drive pro tumorigenic systemic neutrophil-dominant inflammation

Fig. 4: Gemcitabine induces bone marrow myeloid progenitors in a preclinical setting in vivo.The alternative text for this image may have been generated using AI.

a Schematic illustrating the isolation and immunomagnetic enrichment of bone marrow hematopoietic progenitors for downstream single cell RNA sequencing (scRNAseq) analysis (Gemcitabine: 120 mg/kg, Cisplatin: 6 mg/kg, n = 2 mice per group). Created in BioRender. Wong, S. (2026) https://BioRender.com/6i8hwv9. b UMAP projection of eight distinct hematopoietic progenitor cell clusters with colored inference by specific cell type (n = 2 mice per group, 38,675 number of total cells analyzed). c Violin plot and d UMAP projection of hematopoietic progenitor cell clusters colored-based on their normalized expression of cluster-specific genes (Meis1, Gata1, Mt1, Mpeg1, Skap1, Ms4a2, Ms4a3, S100a8 and Ighm). e UMAP projection of hematopoietic progenitor cell clusters isolated from tumor-bearing mice treated with gemcitabine-cisplatin chemotherapy (Chemo) and vehicle (Veh) as a control. f Box plot illustrating the frequency of the GMP population (cluster 6) in tumor-bearing mice treated with gemcitabine-cisplatin chemotherapy and vehicle as a control (n = 2 mice per group). g Frequency of GMPs in the bone marrow of G69 tumor bearing mice treated with vehicle or chemotherapy (n = 5 mice per group) using flow cytometry. Abbreviations: hematopoietic stem and progenitor cell (HSPC), Pro-Erythrocytes (Pro-Ery), monocyte-dendritic progenitor (MDP), common lymphoid progenitor (CLP), basophil-eosinophil-mast cell progenitor (Ba/Eo/MaP), granulocyte-monocyte progenitors (GMP), neutrophil progenitors (NeuP) and pro-B cells (Pro-B cells). Statistics: Two-tailed unpaired t test with Welch’s correction was performed for g. Error bars represent standard error of mean (s.e.m.) unless otherwise stated. Box plots show the minimum and maximum values, and the center line represents the mean. Source data are provided as a Source Data file.

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