Fig. 6: Vitamin A and E supplementation ameliorates CFSS-induced spermatogenic defects and oligoasthenozoospermia in rats.
From: Chronic stress drives oligoasthenozoospermia via gut microbiota-vitamin-Sting1/Slc9c2 axis
a Schematic diagram depicting the experimental design of Vitamin A&E supplementation in CFSS rats. Created in BioRender. Zheng J. (https://BioRender.com/2x9nxx2). b–e Sperm concentration (b), rapid progressive motility (c), progressive motility (d), and total motility (e) of CFSS rats supplemented with vitamin A&E or vehicle control (n = 8 rats per group). f–i Quantitative analysis of Dazl (f, n = 7), Sycp3 (g, n = 7), Tnp1 (h, n = 8), and Pgk2 (i, n = 7) mRNA expression levels in the testicular tissues. j Representative immunofluorescence images of DAZL, SYCP3, TNP1, and PGK2 signals in seminiferous tubules of testicular sections (n = 3 biological replicates per group). Nuclei were counterstained with DAPI (blue). Scale bars = 50 µm. k–n Relative protein expression levels of DAZL (k), SYCP3 (l), TNP1 (m), and PGK2 (n) in the testicular tissues of CFSS rats supplemented with vitamin A&E or vehicle control (n = 4 rats per group). Data are presented as mean ± S.E.M. Two-tailed unpaired t test for (b)–(i), and (k)–(n). The exact p values are presented in the figures as indicated. Source data are provided in the Source Data file.
