Fig. 4: The NTD maintains tRNAAsp with ErdS. | Nature Communications

Fig. 4: The NTD maintains tRNAAsp with ErdS.

From: Structure of ergosteryl-aspartate synthase reveals how an entrapped tRNA is used like a prosthetic swinging arm in the synthesis of aminoacylated sterols

Fig. 4: The NTD maintains tRNAAsp with ErdS.The alternative text for this image may have been generated using AI.

A Alignment of the NTDs from the Afm, Aor, Ncr, and Mor ErdSs. Positively charged residues are blue, residues that interact with the anticodon stem are red, and the region involved in the interaction with the elbow region is highlighted in yellow. B Structure of ErdS with the NTD and tRNAAsp. C,D Close-up view of the contacts between the ErdS N-terminal region and tRNAAsp. E Electrostatic potential of the NTD (blue – basic; red – acidic; white - neutral). F Complementation of the Sce YAL3 dps1 strain with the indicated proteins and ErdS variants. Pictures represent Sce colonies on plates after 3 days of growth. G Complementation of the Sce YAL3 dps1 strain with the indicated Aor ErdS variants. Pictures represent Sce colonies on plates after 3 days of growth. Two clones of the Aor ErdS ΔN90 were used. Arrows indicate positions of several extremely slow-growing colonies.F,Glower panels: TLC profiling of total lipids from these strains to visualize Erg-Asp production (visualization under UV light). Asterisks (*) indicate Erg-Asp bands. Histograms represent the Erg-Asp/Phosphatidylethanolamine (PE) ratio of N = 2 biological replicates.

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