Extended Data Fig. 7: D-bodies are miRNA processing centers that can release miRNA/* duplexes.
From: Phase separation of SERRATE drives dicing body assembly and promotes miRNA processing in Arabidopsis
a, Distribution of pre-miR172b, miR172b/*, BFP-SE, DCL1-GFP and mCherry-HYL1 in pelleted droplet (P) and supernatant (S) fractions before and upon pre-miRNA cleavage. Results from two additional replicates are shown. The relative amounts of the proteins in droplets are determined by densitometry values of the pellet fraction bands relative to that of the unfractionated sample (Total) bands. b, Pull-down results showing the interactions of SE-Flag with DCL1-GFP and HYL1 in the absence or presence of Mg2+. Results from two additional replicates are shown. c, Pull-down results showing the interactions of SE-Flag with DCL1-GFP and HYL1 in the absence or presence of DCL1-GFP. Results from two additional replicates are shown. d, Images showing the incorporation of mCherry-HYL1 into BFP-SE droplets in the presence of pre-miRNA or miRNA/*. Scale bar, 5 μm. Results from two additional replicates are shown. e, Pull-down results showing the interactions of SE-Flag with DCL1-GFP and HYL1 in the absence of RNA or in the presence of pre-miR172b or miR172b/*. Results from two additional replicates are shown. f, Distribution of pre-miR172b, miR172b/* duplex, DCL1, HYL1 and SE in pelleted droplet and supernatant fractions upon pre-miRNA cleavage in the absence or presence of HYL1. Results from two additional replicates are shown. The relative amounts of miR172b/* in the pelleted droplet (P) and supernatant (S) fractions are determined by densitometry values of the pellet and supernatant fraction bands relative to that of the unfractionated sample (Total) bands. g, EMSA results showing the interaction of HYL1 with miR172b/*. Data are representative of 3 independent experiments. h, Pull-down results showing the interaction of HYL1 with miR172b/*. Results from two additional replicates are shown. Uncropped blots for a-c and e-h are provided in Source Data Extended Data Fig. 7.
