Extended Data Fig. 4: Effect of the Atglistatin on lipolysis upon shear stress in KECs.
(a) KEC cells were incubated for 16 h with Red C12, and subjected to flow for 1 day with or without Atglistatin (ATGLi). Cells were labeled with Mitotracker (green) to stain mitochondria and LipidTox to stain LDs (red) and analyzed by immunofluorescence. Scale bars 5 μm. Representative images from n = 3 independent experiments. (b) LD number and total LD area/cell quantified using Icy software from images in a). Data are means ± SEM; n = 3 independents experiments. The statistical significance was calculated by a two-tailed t-test. (c, d) Relative cellular localization of Red C12 (with droplet for c)) or with mitochondria for d)) was quantified by Pearson’s coefficient analysis. Data are means ± SEM. Individual data points correspond to single images analyzed from n = 3 independent experiments. The statistical significance was calculated by a two-tailed t-test. Data are available as source data.
