Extended Data Fig. 10: Meiosis and MII spindle formation in ovulated oocytes of PIWIL3-deficient golden hamsters.
a, PIWIL3w/m and PIWIL3m/m oocytes were collected, and the stages of meiosis at 7 and 10 am in the morning were analyzed following TUBA and DAPI staining. b, MII oocytes at 10 am in the morning were stained with anti-TUBA antibody (green) and DAPI (blue). Scale bars = 5 µm. c, The size of the MII spindles (A and B in Extended Data Fig. 10b) was measured and compared between PIWL3w/m and PIWL3m/m. n indicates the number of independent MII oocytes collected from three females and used for analysis, error bars indicate ± s.d. (A: ***P=0.000417, B: *P=0.0377 by 2-sided t-test).
