Extended Data Fig. 1: Strategy for the production of PIWIL1-deficient golden hamsters by CRISPR/Cas9. | Nature Cell Biology

Extended Data Fig. 1: Strategy for the production of PIWIL1-deficient golden hamsters by CRISPR/Cas9.

From: Production of functional oocytes requires maternally expressed PIWI genes and piRNAs in golden hamsters

Extended Data Fig. 1

a, Structure of the golden hamster PIWIL1 gene and target locus for sgRNA. b, The guide RNA sequence for the golden hamster PIWIL1 gene. c, Production of PIWIL1-deficient golden hamster lines. d, The PIWIL1 nucleic acid and amino acid sequences of the targeted site in wild-type hamsters. Upper case underlined denotes the exon sequence, bold with a red box denotes the target sequence, and red denotes the deleted sequence in the 15Adel4 line. e, The PIWIL1 nucleic acid and amino acid sequence of the targeted site in the mutant line (15 A del4). The amino acid sequences in red indicate sequences changed by deletion.

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