Extended Data Fig. 2: Strategy for the production of PIWIL3-deficient golden hamster by CRISPR/Cas9. | Nature Cell Biology

Extended Data Fig. 2: Strategy for the production of PIWIL3-deficient golden hamster by CRISPR/Cas9.

From: Production of functional oocytes requires maternally expressed PIWI genes and piRNAs in golden hamsters

Extended Data Fig. 2

a, Structure of the golden hamster PIWIL3 gene and target loci for sgRNAs. b, The guide RNA sequences for the golden hamster PIWIL3 gene. c, Production of PIWIL3-deficient golden hamster lines. d, The PIWIL3 nucleic acid and amino acid sequence of targeted site in wild-type hamsters. Upper case underlined denotes the exon sequences, bold with a red box denotes the target sequences, and red denotes the deleted sequence in the 26Adel192 line. e, The PIWIL1 nucleic acid and amino acid sequence of targeted site in the mutant line (26 A del192). The amino acid sequences in red indicate sequences changed by deletion.

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