Extended Data Fig. 3: The small RNA on LDL is predominantly exogenous and removed by LDL re-constitution.
From: LDL delivery of microbial small RNAs drives atherosclerosis through macrophage TLR8
a) Normalized abundance of taxa identified upon alignment of LDL-sRNA to non-host tRNA database (tRNA-db). RPM, reads per million total reads. b) Normalized abundance of bacterial phyla (human microbiome database) contributing sRNA to LDL. c) Normalized abundance of fungal sRNA and representative genomes present on LDL. d) Normalized abundance of algal and protist sRNA and representative genomes present on LDL. RPM, reads per million total reads. Matched nLDL and rLDL samples were fractionated by size-exclusion chromatography (SEC) using two superose-6 columns in tandem and assessed for e) phospholipid and protein content by colorimetric kit (representative data of three independent experiments), f) fluorescence (TopFluor Cholesteryl ester), and g) APOB protein by immunoblot (representative image of three independent experiments). h) Relative expression of exogenous sRNA in matched rLDL and nLDL of a single preparation relative to buffer controls. i) Oil-Red-O staining and fluorescence microscopy (TopFluor Cholesterol ester) (representative images of three biological replicates). Scale bar = 200 μm. Numerical source data, statistics, exact p values and q values are provided.
