Extended Data Fig. 1: nLDL induces inflammatory activation of macrophages.
From: LDL delivery of microbial small RNAs drives atherosclerosis through macrophage TLR8
(a) mRNA expression determined by qPCR of THP-1 macrophages treated with indicated doses of nLDL (matched to Fig. 1f) for 24 h (n = 3 biological replicates). (b) Quantification of immunoblots presented in Fig. 1f (n = 3 biological replicates). (c) Secreted cytokines in the media of THP-1 macrophages stimulated with 0.5 mg/ml nLDL for 3 h, 6 h, 24 h relative to cells receiving no treatment for 24 h (Ctr) (matched to Fig. 1e; n = 3 biological replicates) (d) Primary mouse bone-marrow derived macrophages (BMDM) differentiated with GM-CSF were treated with nLDL (0.5 mg/ml) for 24 h and assayed for mRNA expression by qPCR (n = 3 biological replicates), (e) cytokine secretion by ELISA (n = 3 biological replicates), and (f) protein expression in cell lysates by immunoblot (n = 2 biological replicates). Data are mean ± SEM. (a-c) One-way ANOVA and (d) Two-way ANOVA with Benjamini, Krieger and Yekutieli FDR (Q = 0.05), *q < 0.05, **q < 0.01, ***q < 0.001, ****q < 0.0001. e, Student’s t-test (unpaired, two-sided), **p < 0.01. Numerical source data, statistics, exact p values and q values are provided.
