Extended Data Fig. 8: Gm16685 knockout mouse cancer cells were generated.

a) Schematic view of Gm16685 location in the genome. Gm16685 promoter was highlighted by blue box. NFκB binding motifs in the promoter region of Gm16685 were indicated by yellow box. b) qPCR analysis of Gm16685 expression in GL261-Luc-Gm16685^WT and GL261-Luc-Gm16685^pKO cells with or without TNFα treatment. The data represent means ± s.e.m. of n = 3 biologically independent samples. c) quantification of luminescence intensity obtained from Fig. 6b. n = 6 biologically independent animals. Data are presented as means values +/- SEM. d) Gating strategy of the Flow cytometric analysis for GAMs (CD45+CD11b+). CD45-FITC and CD11b-PE antibodies were used. e) Flow cytometric analysis of GAMs (CD45+CD11b+) from syngeneic mouse model: injecting WT GL261 cells into WT mice (WT-WT), injecting Gm16685 KO GL261 cells into WT mice (KO-WT), injecting WT GL261 cells into KO mice (WT-KO), injecting Gm16685 KO GL261 cells into KO mice (KO-KO). f) Quantification of GAMs (CD45+CD11b+) in those 4 conditions: WT-WT, KO-WT, WT-KO, KO-KO. n = 3. Data are presented as means values +/- SEM. Source numerical data and unprocessed blots are available in source data. p-values: b,c,f, two-tailed Student’s t-test.

Source data