Fig. 6: MIEF1/2 regulate the formation of peri-mitochondrial F-actin and DRP1 foci. | Nature Cell Biology

Fig. 6: MIEF1/2 regulate the formation of peri-mitochondrial F-actin and DRP1 foci.

From: RETRACTED ARTICLE: Mitochondrial mechanotransduction through MIEF1 coordinates the nuclear response to forces

Fig. 6: MIEF1/2 regulate the formation of peri-mitochondrial F-actin and DRP1 foci.The alternative text for this image may have been generated using AI.

a, Co-localization analysis of HA-MIEF1 with endogenous DRP1 along mitochondria (Mito-RFP) in MCF10A-RAS cells upon ROCK–MLCK (YM) treatment for 3 h. Pearson’s indices (below) were calculated based on n = 10 cells per condition from one experiment. Scale bars, 2 μm and 5 μm. b, Quantification of MIEF1 puncta in MCF10A-RAS cells cultured on stiff and soft fibronectin-coated hydrogels (n = 36 cells from two experiments). c,d, Quantification of endogenous DRP1 (c) and MIEF1 (d) puncta in MCF10A-RAS cells with MIEF1/2 knockdown or treated with the CK869 Arp2/3 inhibitor (n = 36 cells in c; n = 21 cells in d; both from two experiments). e, Proximity ligation assay (PLA) between the TOMM20 mitochondrial outer membrane protein and F-actin in MCF10A-RAS cells with MIEF1/2 and DRP1 knockdown or treated with the CK869 Arp2/3 inhibitor (n = 20 cells from two experiments). Scale bar, 2 μm. The data are presented as means and single points. Source numerical data are available.

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