Extended Data Fig. 5: Comparison and trajectory analysis in human CS7 embryo.
From: Spatial transcriptomic characterization of a Carnegie stage 7 human embryo
a. UMAP plot overlaid with PAGA graph showing the developmental trajectory in human CS7 embryonic clusters. b. The scatter-plots showing the expression levels of the changed genes along the velocity pseudotime in lateral plate mesoderm (LP.Meso), axial mesoderm (Axial.Meso) paraxial mesoderm (Para.Meso) and extra-embryonic mesoderm progenitor (EXE.Meso.Prog) lineages. c. UMAP plot showing the cell trajectory in Gast-derived cells along the pseudotime by Monocle3 analysis. d. Heatmap of the percentage cells of embryonic subclusters from Stereo-seq mapped to human cell types at CS7, which suggests our Gast closely resembled their Primitive Steak, our LP.Meso and Axial.Meso closely resembled their Nascent Mesoderm, our Para.Meso closely resembled Emergent Mesoderm, and our EXE.Meso.Prog closely resembled Advanced Mesoderm. e. UMAP visualization of the integrated datasets based on 2000 HVGs of human CS7 embryonic clusters from Stereo-seq and Smart-seq. f. UMAP plot showing the cell trajectory of embryonic subclusters from Stereo-seq and Smart-seq at CS7 along the pseudotime by Monocle3 analysis. g. Ridge plot showing the cell distribution along the pseudotime by Monocle3 analysis.
