Extended Data Fig. 4: The synthesis and detection of acyl-ACP standards via LC/MS. | Nature Cell Biology

Extended Data Fig. 4: The synthesis and detection of acyl-ACP standards via LC/MS.

From: Mitochondrial NADPH fuels mitochondrial fatty acid synthesis and lipoylation to power oxidative metabolism

Extended Data Fig. 4: The synthesis and detection of acyl-ACP standards via LC/MS.

(a) Schematic illustrating workflow for synthesizing acyl-DSL standard for acyl-ACP measurement. (b) The mass spectrometry parameters of various acyl-DSLs. (c-j) The chromatogram of synthesized standards (black) and biological HeLa WT (blue) for Apo-ACP (c), Holo-ACP (d), C2-ACP (e), C4-ACP (f), C6-ACP (g), C8-ACP (h), C10-ACP (i), C12-ACP (j) run on LC/MS.

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