Extended Data Fig. 2: ZNF280A is required for DNA double-strand break repair.
From: ZNF280A links DNA double-strand break repair to human 22q11.2 distal deletion syndrome
(a) RT-PCR analysis of znf280a (ZNF280A) mRNA transcripts in HeLa cells treated with non-targeting or ZNF280A-targeting siRNA sequences. N = 3, error represents S.E.M. (b) Representative images of clonogenic survival assay in HeLa cells, in response to increasing doses (Gy) of ionizing radiation. (c) RT-PCR analysis of znf280a (ZNF280A) mRNA transcripts in U2OS cells treated with non-targeting or two independent ZNF280A-targeting siRNA sequences. N = 3, error represents S.E.M. (d) Representative images of clonogenic survival assay in U2OS cells, in response to increasing doses (μM) of Etoposide. (e) Immunoblot demonstrating knockdown of endogenous ZNF280A protein and over-expression of V5-ZNF280A in U2OS cells. (f) Quantitation of the % of HeLa cells with >10 53BP1 foci, 48 h following 3 Gy ionizing radiation, as depicted in Fig. 2j. N = 3, error represents S.E.M. *p = <0.05, **p = <0.005. two-tailed t-test. (g) Quantitation of the number of chromosomal aberrations per metaphase in untreated cells, similar to that depicted in Fig. 2l, m. N = 3; error represents S.E.M. Source numerical data and unprocessed blots are available in source data.
