Extended Data Fig. 3: Dietary galactose prevents tumour-infiltrating CD8+ T cell exhaustion. | Nature Cell Biology

Extended Data Fig. 3: Dietary galactose prevents tumour-infiltrating CD8+ T cell exhaustion.

From: Diet-derived galactose reprograms hepatocytes to prevent T cell exhaustion and elicit antitumour immunity

Extended Data Fig. 3: Dietary galactose prevents tumour-infiltrating CD8+ T cell exhaustion.

a, Cell numbers of tumour-infiltrating CD8+ T cells from B16-F10 or LLC tumour-bearing C57BL/6 WT mice fed a high-galactose diet or normal diet. Tumour-infiltrating CD8+ T cells were isolated at day 14 after tumour injection. Cell number was normalized to 1 g tumour tissue (n = 7 mice). b, Flow cytometric analysis of the proportion and numbers of tumour-infiltrating IFN-γ+CD8+ T cells and Ki-67+CD8+ T cells from B16-F10 tumour-bearing C57BL/6 WT mice on a high-galactose or normal diet (n = 7 mice). c, Proportion of tumour-infiltrating Ki-67+ (n = 7 mice) and IFN-γ+ (n = 5 mice) CD4+ T cells from B16-F10 tumour-bearing C57BL/6 mice on a high-galactose or normal diet. d, Flow cytometric analysis of proportion and numbers of tumour-infiltrating PD-1+TIM-3+, TCF-1+TIM-3− CD8+ T cells from LLC tumour-bearing C57BL/6 WT mice fed a high-galactose diet or normal diet. Cell number was normalized to 1 g tumour tissue (n = 7 mice). e,f, Flow cytometric analysis of the proportion of tumour-infiltrating IFN-γ+CD8+ T cells (e, n = 8 mice), Ki-67+CD8+ T cells (n = 7 mice), IFN-γ+CD4+ T cells and Ki-67+CD4+ T cells (f, n = 6 mice) from LLC tumour-bearing C57BL/6 WT mice on a high-galactose or normal diet. g, Tumour size of B16-F10 tumour-bearing C57BL/6 WT mice. C57BL/6 WT mice were injected with B16-F10 tumour cells and then fed with normal control diet (n = 12 mice) or high-galactose diet (n = 13 mice). h, Flow cytometric analysis of proportion of PD-1+TIM-3+, TCF-1+TIM-3− CD8+ T cells from tumours of B16-F10 tumour-bearing mice from g (Ctrl, n = 7 mice; Gal, n = 8 mice). i, Flow cytometric analysis of proportion of IFN-γ+CD8+, Ki-67+CD8+, Ki-67+CD4+ T cells from tumours of B16-F10 tumour-bearing mice from g (n = 8 mice). The experiments were independently repeated two times. Data are presented as mean ± SEM. P values were calculated by two-tailed Student’s t-test (a-f,h,i) and two-way ANOVA with Geisser–Greenhouse correction (g). ns, not statistically significant.

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