Extended Data Fig. 7: Impact of TMEM65 mutations on mito-NCX function.
From: TMEM65 functions as the mitochondrial Na+/Ca2+ exchanger
WT or mutant TMEM65 constructs were expressed to comparable levels in TMEM65-KD HEK cells as shown in Western blot images. Control: untransfected cells. Data are presented as mean ± SEM. Statistical analysis was performed using unpaired, two-tailed t-test. Actin: loading control. Molecular weight marker unit: kDa. All conditions (WT, Control, E144A, S154A, and T155A) were analysed with 3 independent biological replicates.
