Fig. 3: Enhancer connectomes linking glioma-associated risk SNPs to target genes.
From: Systematic decoding of functional enhancer connectomes and risk variants in human glioma
a, The genomic distribution of glioma-associated risk SNPs. b, Proportions of SNPs located in non-coding regions with or without H3K27ac modifications. c, Luciferase reporter assays reveal differential enhancer activity in glioma cells between risk and non-risk alleles for 12 risk SNP-associated enhancers. Top: the schematic of the luciferase reporter used to detect enhancer activity. Bottom: bar plots represent mean ± s.d. luciferase activity from three independent biological replicates. d, A scatter plot showing SNP-associated enhancers ranked by log2 fold change in sgRNA enrichment from CRISPRi screening. e, CRISPRi with rs2297440-associated enhancers significantly inhibits glioma cell proliferation. Data represent mean ± s.d. from three independent biological replicates. f, The interaction profile of an enhancer carrying risk-associated SNP rs2297440 and its target genes. g, Enrichment of target genes interacting with SNP-associated enhancers in glioma cells, as identified by CRISPRi screening. Statistical significance was determined by two-sided t-test. NS, not significant.
