Fig. 5: Overexpression of SOX18 in eKO cells restores self-renewal in glioma cells.
From: Systematic decoding of functional enhancer connectomes and risk variants in human glioma
a, MTT assay showing increased proliferation of enhancer KO (eKO) cells upon overexpression of SOX18. b, Colony formation assay demonstrating that SOX18 overexpression restores the colony-forming ability of eKO cells. c, GSC sphere models formed by WT, eKO and SOX18-overexpressing eKO cells. Scale bar, 100 μm. d, Quantification of GSC sphere formation in c. e, In vivo bioluminescence imaging of xenografts derived from WT, eKO and SOX18-overexpressing eKO cells at days 9, 18 and 27 post-implantation. f, Quantification of in vivo bioluminescence signals in e. Data represent mean photon flux ± s.d. from five independent mice per group. g, Cell proliferation assay in SOX18-KO U251 cells. h, Colony formation assay in SOX18-KO U251 cells. Unless otherwise specified, data are presented as mean ± s.d. from three independent biological replicates. Statistical significance was determined by two-sided t-test. NS, not significant.
