Fig. 6: The risk allele of rs2297440 (C/C) promotes SOX18 expression and glioma progression.
From: Systematic decoding of functional enhancer connectomes and risk variants in human glioma
a, A schematic showing programmable editing of rs2297440 in U251 cell with a dsDNA donor. b, 3C-qPCR analysis showing the interaction frequency between the SOX18 TSS and the rs2297440 locus in WT and single-base edited mutant (MUT1 and MUT2) U251 cells. c, MTT assay showing the proliferation of WT cells and mutant cells over time. d, Quantification of colony formation in WT and mutant U251 cells. e, RT-qPCR analysis for SOX18 expression in U251 WT and mutant cells. f, The number of colonies formed by mutant cells with or without overexpression of SOX18. g,h, MTT assay assessing the proliferation of mutant U251 cells with or without SOX18 overexpression. i,j, GSC sphere formation assay evaluating the tumour-forming capacity of WT and mutant U251 cells, with and without SOX18 overexpression. Representative images of spheres (i). Scale bar, 100 μm. Bar plot quantifying the number of spheres (j). Data represent mean ± s.d. from three independent biological replicates. Statistical significance was determined by two-sided t-test. NS, not significant.
