Fig. 7: regulates SOX18 expression through allele-specific recruitment of MEIS1 binding.
From: Systematic decoding of functional enhancer connectomes and risk variants in human glioma
a, Motif analysis of rs2297440-associated enhancer, with relative scores obtained from the JASPAR database. b, ChIP–qPCR detection of MEIS1, SOX18 and PAX5 binding to rs2297440 locus between risk (WT) and non-risk (single-base editing) allele. IgG served as the negative control. c, SOX18 mRNA expression level in U251 cells after MEIS1 overexpression using dCAS9–CRISPRa. d, SOX18 mRNA expression level in U251 cells after MEIS1 overexpression using an exogenous overexpression plasmid. e, A schematic illustration of how rs2297440 allele modulates MEIS1 binding and regulates SOX18 expression and glioma progression. Data represent mean ± s.d. from three independent biological replicates. Statistical significance was determined by two-sided t-test. NS, not significant. TF, transcription factor.
