Extended Data Fig. 5: Depletion of CD8 T cells enhanced the growth of transplanted tumor cells.
a, EC-specific deletion of Parp1 increased CD8+ T cell number in the presence of CBP. CD8 T cell number (per mg of tumor) was quantified by flow cytometry. Each dot represents one individual animal (n = 8 biological replicates). b, Flowchart of CD8+ T cell depletion by anti-CD8a antibody in E0771 tumor-bearing mice. Depletion of CD8+ T cells was achieved by intraperitoneal injections of 100 μg of anti-mouse CD8a antibody suspended in 200 μL of PBS every 4 days, starting one day before tumor cell injection. Depletion of CD8 T cells was confirmed by flow cytometry analyses of peripheral blood at day 5 and day 13. c, Flow-cytometry plots of CD8+ populations in peripheral blood at day 5 and 13 demonstrate that CD8+ T cell population was depleted by αCD8 antibody (n = 8 biological replicates). d, e, Breast tumor tissues were excised from Sdf1iΔEC/iΔEC or control Sdf1loxP/loxP mice and analyzed with flow cytometry (d). Percentage of CXCR3+CD8+ T cell population in the retrieved CD8+ T cells from indicated mice was compared (e). Each dot represents sample from individual animals (n = 8 biological replicates). **P < 0.01, ****P < 0.0001 and N.S. were calculated using two-sided unpaired t-test for a or two-way ANOVA followed by Tukey’s test as post-hoc analysis for c and e.
