Extended Data Fig. 4: Nox1- and Npy1r-GFP reporters recapitulate the endogenous expression seen in the small intestine and bladder.
From: NOX1 and NPY1R mark regional colon stem cell populations that serve as cancer origins in vivo
a) GFP IHC performed on Nox1-2A-EGFP mouse line was observed in the +4 to +6 presumptive TA cells and the CBC cells in the ileum at a lower level. This expression was detected at stronger level in the same cells of the crypt bordering the peyer’s patch. Green arrows indicate the +4 cell position while red stars indicate the CBC cell position (n = 5); b) Co-IF of GFP with the epithelial marker E-cadherin, the mesenchyme marker Vimentin, and the muscle marker a-SMA showed that GFP+ cells were Vimentin+ and excluded from the urothelium and muscle cells (n = 5). Scale bar: 25μm (a) and 50μm (b).
