Extended Data Fig. 7: A subset of Nox1-expressing cells in the small intestine is self-renewing and multipotent.
From: NOX1 and NPY1R mark regional colon stem cell populations that serve as cancer origins in vivo
a) Nox1-2A-CreERT2, Rosa26-LSL-tdTomato mice were injected with 3 mg/30 g body weight of tamoxifen and harvested after 24 h, 1-week, and 1-month. At 24 h, majority of the cells in the vicinity of the +4 cell were RFP +. Only a small subset of CBC cells were RFP + . After one month of tracing, the crypts and villi in the ileum were partially covered by RFP-expressing cells. Red asterisks represent RFP + CBC cells; b) Frequency of RFP+ cells increased in the crypts adjacent to the Peyer’s patches after 24 h induction. All cells within the crypt-villus units adjacent to the Peyer’s patches were RFP+ at 1-month. Scale bar: 25μm.
