Extended Data Fig. 8: Npy1r is expressed in long-lived stromal cells of the bladder.
From: NOX1 and NPY1R mark regional colon stem cell populations that serve as cancer origins in vivo
a) Graph representing the percentage of crypts retaining the RFP label for each tissue compartment (CM: 22.67% ± 3.53%; CD: 74.79% ± 4.40%; Rectum: 81.79% ± 4.04%; n = 3) at one month post-induction in adult Npy1r-EGFP-ires-CreERT2, LSL-TdTomato mice; b) Npy1r- EGFP-ires-CreERT2, Rosa26-LSL-tdTomato mice were injected with 4 mg/30 g body weight of tamoxifen and harvested after 24 h (n = 3), 1-week (n = 3), and 1-month (n = 3). At 24 h, RFP was detected in stromal cells. This pool of cells was maintained 1 week and 1 month after induction; c) Co-IF of RFP with the epithelial marker E-cadherin, the mesenchyme marker Vimentin, and the muscle marker a-SMA showed that RFP+ cells were Vimentin+ and excluded from the urothelium and muscle cells (n = 3). Scale bar: 50μm.
