Extended Data Fig. 10: Npy1r CreERT2-driven Apc deletion does not initiate tumour formation in the bladder.
From: NOX1 and NPY1R mark regional colon stem cell populations that serve as cancer origins in vivo
a) 3-month old Npy1r-EGFP-IRES-CreERT2, APCfl/fl mice were harvested and stained for RFP in the entire colorectum to evaluate any potential Cre recombinase expression leakage. No RFP staining was detected (n = 3); b) Npy1r-EGFP-IRES-CreERT2, APCfl/fl mice were injected with 3 doses of 4 mg tamoxifen per 30 g body weight every 2 days and harvested 8 weeks post- induction. No β-catenin accumulation was detected in the bladder (n = 6). Scale bar: 500 μm.
