Fig. 2: NOX1 and NPY1R are enriched in colon stem cell clusters.
From: NOX1 and NPY1R mark regional colon stem cell populations that serve as cancer origins in vivo
a, Heatmap representing the level of expression of identified key lineage markers for each cluster in the caecum and proximal, middle and distal colon. b, UMAP plots representing all of the clusters identified (left) before being reassigned to different lineage identities using the expression heatmap in a (right). c, UMAP plots representing the distribution of LGR5+ (top), NOX1+ (middle) and NPY1R+ cells (bottom) for each colonic region (caecum and proximal, middle and distal colon). The stem cell (SC) cluster is delineated by a blue dotted line, the enteroendocrine (EE) cluster is delineated by a brown dashed line and the tuft cluster is delineated by a purple dashed line, according to the expression of consensus lineage markers as defined in b. Assays were performed on one biological sample per tissue region. CM, middle colon; DCS, deep crypt secretory.
