Fig. 3: NOX1 and NPY1R are enriched at the base of colonic crypts.
From: NOX1 and NPY1R mark regional colon stem cell populations that serve as cancer origins in vivo
a, RNAscope analysis of Lgr5, Nox1 and Npy1r in the different regions of the mouse colon, revealing enrichment of NOX1 and NPY1R at the crypt base of all compartments and the middle and distal colorectum, respectively (n = 5). b,c, Nox1-2A-eGFP (n = 5) (b) and Npy1r-eGFP-IRES-CreERT2 (n = 5) (c) mouse models were generated by incorporating the 2A-eGFP cassette before the stop codon at the Nox1 gene locus and the eGFP-IRES-CreERT2 cassette after the start codon at the Npy1r gene locus. GFP IHC recapitulated endogenous NOX1 and NPY1R expression, as seen in a. GFP expression was also detected in the base of rectal crypts in Npy1r knock-in mice. Scale bars, 25 μm.
