Fig. 5: NOX1-expressing colon cells are self-renewing, multipotent stem cells in vivo. | Nature Cell Biology

Fig. 5: NOX1-expressing colon cells are self-renewing, multipotent stem cells in vivo.

From: NOX1 and NPY1R mark regional colon stem cell populations that serve as cancer origins in vivo

Fig. 5: NOX1-expressing colon cells are self-renewing, multipotent stem cells in vivo.

a, A Nox1-2A-CreERT2 mouse model was generated by incorporating the 2A-CreERT2 cassette before the stop codon at the Nox1 gene locus. Mice were crossed with the Rosa26-LSL-tdTom mouse line. b, Mice were injected with 3 mg tamoxifen per 30 g body weight (3 mg/30 g) and analysed at 24 h (n = 3), 1 week (n = 3) and 1 month (n = 3) after induction. RFP IHC showed lineage tracing of NOX1-expressing cells and their cell progeny. c, Mice were injected at a lower tamoxifen dose (0.1 mg per 30 g body weight) and harvested at 1 month (n = 3). Co-IF of RFP and the lineage markers MUC2, CHGA and DRA showed that all RFP+ traced units expressed major lineage markers. Scale bars, 25 μm.

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