Extended Data Fig. 7: Leucine effect on mitochondrial proteome.
a. Percentage of proteins increased or decreased in whole worm proteome, MitoCarta 3.0 proteins, and OMM proteins upon leucine treatment. b-c. Volcano plots showing changes of OMM proteins (b) and whole worm and MitoCarta 3.0 proteins (c) upon leucine treatment. P values were determined by unpaired two-tailed t-test. OMM (b) and mitochondrial (c) proteins show the tendency to be more abundant in leucine-treated worms d. Log2 fold change (FC) of whole worm proteome, MitoCarta 3.0 proteins, and OMM proteins comparing Leu with dH2O control treatment. Two-tailed Mann-Whitney U test between whole worm proteins and other protein groups. e. Enriched GO terms (p < 0.001 and FDR < 0.2 cutoff) for upregulated proteins of worm mito proteome upon leucine treatment. f-g. Volcano plot showing changes of OMM proteins (f) and whole worm and MitoCarta 3.0 proteins (g) upon leucine treatment. h. Volcano plot showing changes of whole worm and MitoCarta 3.0 proteins upon CHX treatment. i-j. Volcano plot showing changes of OMM proteins (i) and whole cell and MitoCarta 3.0 proteins (j) upon leucine treatment. k. Volcano plot showing changes of whole cell and MitoCarta 3.0 proteins upon CHX treatment. P values were determined by unpaired two-tailed t-test (f-k). Source numerical data are available in Source data.
