Extended Data Fig. 10: Additional evidence supporting the link between BCAA catabolism and OMM proteostasis in fertility and cell proliferation under OMM perturbation. | Nature Cell Biology

Extended Data Fig. 10: Additional evidence supporting the link between BCAA catabolism and OMM proteostasis in fertility and cell proliferation under OMM perturbation.

From: Leucine inhibits degradation of outer mitochondrial membrane proteins to adapt mitochondrial respiration

Extended Data Fig. 10

a. Design of bcat-1(E279K) CRISPR editing strain. b. Whole worm imaging of mitoUFD in WT and bcat-1(E279K)/+ worms. Scale bar = 10 µm. c. Egg hatch rate for Fig. 5l. n = 15 (WT_L4440), 14 (WT_gcn-2 RNAi), 15 (bcat-1/ + _L4440), 14 (bcat-1/+_gcn-2 RNAi) animals. d. Egg hatch rate for Fig. 5m. n = 15 (SCR control), 16 (gcn-2 OE) animals. e. Information of lung cancer cell lines from DepMap. f. Cell viability of HEK293 over four days with 0 µM, 4 µM and 10 µM MitoBlock-12 (MB-12) treatment. Two-way repeated ANOVA. Mean ± sem, n = 3 independent biological replicates with 3 technical replicates each day each condition. g-j. Cell images of HEK293 (g), H2030 (h), H1437 (i) and H1666 (j) at day 2 and day 4 after seeding. 3000 cells seeded on day 0 for HEK293, H2030 and H1437, 6000 cells seeded on day 0 for H1666. Source numerical data are available in Source data.

Source data

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