Extended Data Fig. 3: RNAi screen for genes involved in amino acid metabolism, amino acid transport, glucose sensing and metabolite transport.
a. RNAi screen with the mitoUFD substrate for genes encoding enzymes for amino acid metabolism. Fluorescence in worms was quantified by worm sorter. 50-250 animals were measured for each gene. b. RNAi knockdown in the mitoUFD strain for genes involved in amino acid transportation, glucose sensing, and metabolite transportation at IMM. Mean ± sem, n = 4 independent biological replicates with total animal number of 24-32. ImageJ quantification. One way ANOVA with Dunnett correction for multiple comparisons. c. For the genes that stabilized mitoUFD of more than 20% in the targeted RNAi screen for 135 amino acid metabolic genes, the involved amino acids, ratio (hits/total genes per category) and their stabilization scores are shown based on pathway analysis. d. RNAi knockdown in the mitoGFP strain for genes involved in leucine sensing. Mean ± sem, n = 4 independent biological replicates with total animal number of 24-32. ImageJ quantification. One-way ANOVA with Dunnett correction for multiple comparisons with L4440 RNAi control. Source numerical data are available in Source data.
